SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 5. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). , Sunnyvale, CA). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 306 plate air cal fail,low light. 1. 0200-6117. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. ). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Supplier: Molecular Devices. Wavelength range: 340-850 nm. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax Multi-Mode Microplate Readers. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. Molecular Devices SpectraMAX 340 Microplate Reader. Read one sample or up to 384. SpectraMax Plus 384 measuring instruments pdf manual download. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. using SpectraMax i3 (Molecular devices, San Jose, USA). Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Molecular Devices Spectramax 340PC instructions. 30. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. , Fremont, CA, USA). 303 unable to cal dark current. Features showcased by this model include. 5. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. These lab workhorses are used in drug discovery, research, bioassay validation, and. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 313 reference gain check fail. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 6. Values were determined according to a standard curve (log/log curve-fit). SpectraMax® Microplate Readers and SoftMax® Pro Software. View the Molecular Devices website. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). 2. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. ), which was proportional to the number of damaged cells. 306 plate air cal. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. edu Contact Phone: 216-368-8962. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Molecular Devices’ PathCheck® sensor technology. Crowder, Ziqian Zeng, Alissa N. Molecular Device M5e. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. Molecular Devices SpectraMax M5e Microplate Reader. Number of flashes. 190-1000 nm, tunable in 1 nm increments. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. , Sunnyvale, CA). SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The plate was covered and incubated for one hour at room temperature. Donor: 620 nm. Quick View. No power cord or accessories included. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. control. Our absorbance plate readers feature our PathCheck Sensor technology and. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Dissolve 13. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SpectraMax® Microplate Readers and SoftMax® Pro Software. Pharmacokinetic and pharmacodynamic analysis. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 0 3. Analysis of ALA by Ehrlich’s Reagent Assay. Unit has several areas of damaged plastic. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. N-34-LB. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 5. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Call 1-800-745-2710. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. 30 µs. reader (SpectraMax 340; Molecular devices, LLc). Molecular Devices Corporation 1311. Water – distilled water or equivalent. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Cell permeabilization. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Service Contracts, Preventative Maintenance and Performance. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Call 1-800-745-2710. TDP-43 stress granular cells were treated with 70% ice-cold. SpectraMax Microplate Readers. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. This assay measures the two major sugars in wine, glucose and fructose. 314 low lamp level warning. They are for reference only. 0 to 4. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 30. Donor: 616/10 nm. Data were expressed as the mean percent viable cell vs. Samples with OD values of less than 0. Acceptor: 665nm. 0% and ± 0. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. 4. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Manager: Yinghua Chen, PhD Contact Email: yinghua. Cross sections of mouse skin was prepared and stained as previously described . The plate was covered and incubated for one hour at room temperature. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Microplate Spectrophotometer Operator’s Manual. Stat Fax 3200 Microplate Reader. Acceptor: 665/10 nm. D2. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Operators can conveniently select up to 6 wavelengths at a time for. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Streamline GLP/GMP compliance. Donor filter: 616/10 nm. Example: [Drawer]. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 001/min at 425 nm. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. 3. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Please see photos. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Quick View. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. 4) was followed according to Vanacker et al. chen@case. To measure the cell biomass (g/L),. 024). Unit powers. Alkaline phosphatase (ALP) activity. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). application or budget. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. SpectraMax 384 Plus. Description. Lactate Dehydrogenase (LDH) Release. 316 grating motor driver faulty. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. These models can no longer be repaired. The ratio of red to green fluorescence intensity was calculated. Microplate Readers. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 313 reference gain check fail. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. 20. . The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). used in the SPECTRAmax PLUS. gp70 blocking. One plate carrier adapter is provided with the instrument. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 305 reference level saturation. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Statistical analysis. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The slope of the linear portion of the kinetic curve generated from each well was used to. SPECTRAmax® 190. Or scan up to 384 wells at any time to determine the best assay conditions. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. SpectraMax 340 from Molecular Devices). The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. AoSMCs were seeded into 6-well culture plates. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. 315 can't find zero order. Molecular Devices SpectraMax Gemini XS Microplate Reader. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 0200-0729. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. This was followed by an additional incubation at 37 °C for 30–60 min. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Details. more on but is otherwise untested and sold AS-IS. Lactate Dehydrogenase (LDH) Release. Labsystems Luminoskan RT Microplate Reader. Microplate Spectrophotometer Operator’s Manual. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Driessen*,MaaikeA. 9000-0756. , San Jose, CA) using Softmax Pro 7 software. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. SpectraMax Multi-Mode Microplate Readers. 6) and 0. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . SpectraMAX 340 plate reader (Molecular Devices). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The loading equaled the mass of BSA per mass of particles. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. TGFα release was calculated as described in the Results. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). 2. 190–850 nm <± 1. The chamber contains. The Catalina. Molecular Devices SpectraMax Gemini XS Microplate Reader. Page: 1/6. Not. , Sunnyvale, Calif. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). 5 ng/ml; estradiol: 10 pg/ml. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. Warranty. MMP‑1 inhibition assay. Cell proliferation was measured using the MTT method. #N207) LAL Reagent Water (Lonza cat. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 04. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). SpectraMax Multi-Mode Microplate Readers. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Money Saved: $340. 3 mm. A. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. Incubation with TMB substrate was done for 18 min. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Products : SpectraMax Plus 384 reader,. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. In this study we used tetanus toxoid (TT) as a well-defined antigen to. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 1% N-1-naphtylethylenediamine dihydrochloride, 2. SpectraMax 190 reads 96-well and strip-well plates. 0, MSD) or Spectramax 340 (Molecular Devices). 5. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Other technologies such as imaging. Upgradeable modules are also available including western blot, cell imaging, and fast. 0, 20 mmol L –1cal-cium chloride). , Sunnyvale, CA). For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 5 mL sample or standard solution was well mixed with 0. 5% phosphoric acid) in microplates at room temperature for 10 min. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Details. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. SpectraMAX 340. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Wavelength Range. Molecular Devices SpectraMax M5e Microplate Reader $26,500. 0 OD. S. Each experiment was performed in duplicate. A 0. ). TDP-43 stress granular cells were treated with 70% ice-cold. SpectraMax 190 reads 96-well and strip-well plates. Manager: Yinghua Chen, PhD Contact Email: yinghua. Molecular Device M2e. 2. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. , Fremont, CA, USA). Intra- and inter-assay coefficients of variation were lower than 8. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. The absorbances were being measured at 515 nm. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. 5 mL of 1 M acetate buffer (pH 4. . Cell culture 3T3 fibroblasts and RAW 264. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Homeostatic model assessment for insulin. g. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. , Sunnyvale, CA, USA) at 595 nm. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The reaction mixture was incubated for 5 min. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 00 USD. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. VersaMax reads 96-well and strip-well plates. Binding affinity for denosumab. 1 mL of culture media with 10% FBS. Microscopy. Molecular Devices SpectraMax M5e Microplate Reader. The SpectraMax® iD3 Multi Mode Microplate . 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. 2. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. SpectraMAX 340. Details. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Clotting times (mean ± SD) for rSimukunin and rSV. #N207) LAL Reagent Water (Lonza cat. 4. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Molecular Devices. . Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The VersaMax and SpectraMax 190 read 96-well microplates. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Stat Fax 4700 Microstrip Reader. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Bertin Precellys Evolution Touch Homogenizer. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood.